Dr. Revathy Sekaran

Field of interest

Effective utilization of bioactive compounds from natural sources for development of drugs useful for cancer chemotherapy. As drug resistance is the major impediment in cancer therapy, reversing the drug resistance using traditional and natural compounds to improve effective treatment for cancer.

Research and Training Experience

Internship - thesis: “Analysis of Human metaphase chromosome using GTG banding technique – A case study of Turner syndrome” Under the guidance of Dr. Solomon F.D. Paul, Professor and Head, Dept of Human Genetics, Sri Ramachandra Medical College and Research Institute, Chennai-116.

Experienced on handling microscope, maintaining tissue culture, slide preparation for karyotyping, isolation of DNA.

M.Sc thesis: “Phenotypic detection of high-level aminoglycoside resistance (HLAR) in Enterococcus species in a tertiary care centre” Under the guidance of Dr. K.S. Sridharan, Assistant professor, Dept of Microbiology, Sri Ramachandra Medical College and Research Institute, Chennai-116.

Experienced in
• Handling and Maintenance of microbial cultures
• Training in microscopy
• Detection of microbial drug resistance by disk diffusion and agar dilution methods.

PhD thesis: “Studies on modulation of MDR-1 gene expression by curcuminoids from Curcuma longa L. in Multidrug Resistance KBChR8-5 Human Cervical Cancer Cell Line”. Under the guidance of Dr. S. Elumalai, Head of the department, Department of Biotechnology, University of Madras, Chennai-5.

My research work included handling and maintenance of animal cell culture, with hands-on training on:
• Gradient, and real time-PCR
• Agarose gel electrophoresis
• RNA isolation
• cDNA preparation
• Cell cycle analysis
Analytical techniques:
• Extraction of compounds from plants
• Chromatography techniques including TLC, Column Chromatography, HPLC, GC-MS.

Trainings and workshops attended

• National workshop on “Methods in In-vitro Toxicology” at Bharathidasan University, Trichy, 2010 and 2011.
• Training course on “Nanoscience and Nano-biotechnology” at Karunya University, Coimbatore, 2010.
• Training programme in Biomedical Laboratory Sciences in the SNONGC Department of Genetics and Molecular Biology, 2008, at Vidyasagar Institute of Biomedical Technology and Science (VIBS), Chennai.
• Project training on “Cytogenetic Karyotype Blood Investigation” at Dept. of Genetics Sri Ramachadra Medical College and Research Institute, Porur, Chennai, 2007.
• Training in plant tissue culture on Cardamom and Vanilla at Indian Spices Board Laboratory in Kochi, 2007.




Research articles published



• Effect of curcuminoids on MDR-1 gene expression in multidrug resistance KBChR-8-5 human cervical cancer cell line. Journal:- Int. J. Pure App. Biosci. Vol 4 (4): 229-237. [International Journal of pure and applied Bioscience] S.Revathy, S. Elumalai, 2016.
• Invitro Evaluation of anti-cancer activity of curcuminoids from turmeric (curcuma longa.L) against multidrug resistant tumor cell lines. Journal:- Int.J.Adv.Res Vol 4(8), 205-215. [International Journal of Advanced Research] S.Revathy, S. Elumalai, 2016.
• Study of Collateral Sensitivity of Multidrug Resistant Cell Lines to Curcuminoids. Journal:- Int.J.Curr.Microbiol.App.Sci. Vol 2(5):28-39. [International Journal of Current Microbiology and Applied Sciences] S.Revathy, and S.Elumalai 2013.
• Isolation, Purification and Identification of Curcuminoids from Turmeric (curcuma longa L.) by Column Chromatography. Journal:- Journal of Experimental Sciences. Vol. 2(7): 21-25. S.Revathy, S. Elumalai, Merina Benny and Benny Antony, 2011.
• Evaluation of Curcuminoids in Turmeric Rhizome (curcuma longa L.) collected from different places in India. Journal:- Biosciences, Biotechnology Research Asia. Vol. 8(1), 259-264. S.Revathy, S. Elumalai, Merina Benny and Benny Antony, 2011.
• Phenotypic detection of High-Level aminoglycoside Resistance (HLAR) in Enterococcus species in a tertiary care centre. Journal:- Biosciences, Biotechnology Research Asia. Vol. 6(2), 751-756. S.Revathy, S. Elumalai, 2009.




Paper presentation



• International Conference on Natural Products and Biomedical Technology (ICNPBT), Annamalai University, Chidambaram, Tamil Nadu, India.




Leadership



• Trained two M.Sc. students and one M.Tech. student in the field of cancer biology and molecular biology for their dissertation projects, 2012 - 2013.




Conferences attended



• National seminar cum workshop on “Applied Plant Genomics”, 2012, Presidency College (autonomous), Chennai.
• International conference on “Frontiers in Pharmaceutical Chemistry & Biologics - An Interdisciplinary Approach”, 2011, Women’s Christian College, Chennai.
• International summit on “Exploring the secrets of Bio-fuel”, 2011, Presidency College (autonomous), Chennai.
• International conference on “Natural Products and Biomedical Technology (ICNPBT)”, 2011, Annamalai University, Chidambaram, Tamil Nadu, India.
• International conference on “Cancer Biology: Molecular Mechanisms and Novel Therapeutics”, 2010, IIT Chennai.
• Conference on “Effective control of Emerging & Re-emerging Viral Diseases”, 2010, King Institute of Preventive Medicine & Research, Chennai.
• National conference on “Recent Advances in Herbal Drug Processing, Development and Research”, 2010, Sri Ramachadra Medical College and Research Institute, Chennai.
• “Recent Trends in Nano – Biotechnology and Nanotechnology Instrumentation”, 2009, IIT, Chennai.
• “Second Annual Science Symposium on HIV/AIDS”, 2009, YRG CARE, Taramani, Chennai.
• National Level Symposium on “Biotechnology – A Future Perspective”, 2008, SRM University, Chennai.
• “CMC - NIV Symposium, Continuing Medical Education”, 2008, Christian Medical College, Vellore.
• National conference on “Recent trends in Biochemistry and Biotechnology – with special reference to Natural Products in Health”, 2006, Annamalai University, Chidambaram.
• National conference on “Natural Products in Health”, 2005, Annamalai University, Chidambaram.




Conference organized



• “Recent Trends in Nano – Biotechnology and Nanotechnology Instrumentation”, 2009, IIT, Chennai.
• International summit on “Exploring the secrets of Bio-fuel”, 2011, CLRI, Chennai.
• National seminar cum workshop on “Applied Plant Genomics”, 2012, Presidency College (autonomous), Chennai.
• Active participant in NSS, a volunteer of unit XXVII Annamalai University, Chidambaram, Tamil Nadu, 2005 -2006.
• Organized and participated in special seminar on “women empowerment” Annamalai University, Chidambaram, Tamil Nadu, 2006.
• Organized and participated in special seminar on “youth and Discipline” Annamalai University, Chidambaram, Tamil Nadu, 2005.

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