Comprehensive and in-depth knowledge in vascular biology, Redox Biology ,cardiovascular and stem cell biology. Developed techniques to isolate beating cardiomyocytes from rat pups and mastered isolation of stem cells from Umblical Cord, Bone marrow and cardiac explants.
Developed a CAM model to study angiogenesis and has developed Hypoxia models to study various therapeutic targets.
Discovered the anti-apoptotic property of EETS, leading to the development of possible drugs for myocardial Ischemia.
Areas of Expertise
• Tissue Engineering
• Angiogenesis
• Cell signalling
• Hypoxia
• Stem cell Technology
• Cardiovascular Therapy
• Cancer Biology
Stem cell Technology
• To create a platform that fosters inter-disciplinary collaborations toward bench to bedside translation of stem cell technologies for heart and vascular disease management
• To study the role of Wnt signaling in Stem cell differentiation
• Role of conditioned media from MScs
• Effects of micro-environmental stress on the regenerative activity of mesenchymal stem cells.
My lab focuses on further understanding of stem cell behavior, characterization, manipulation, expansion and therapeutic delivery
Cardiovascular Therapeutics
• In vitro studies in enhancing CSCs survival, proliferation and subsequent differentiation to functional Cardiomyocytes under hypoxic conditions.
• Analysis of anti-apoptotic and cyto protective pathways give clue regarding molecular mechanisms involved in decreasing the extent of cell death.
• Isolation, culture and characterization of CSCs from rat heart biopsies by explant method
Cancer Biology
• Understanding the signaling mechanisms in Cancer stem cells
• Mitochondrially targeted compounds novel therapeutic agents for Cancer novel therapeutic agents for Cancer
• Tumour Angiogenesis
Our goal is to understand the mechanism of tumor derived cytokines recruits and regulates the fate of infiltrating stem cells and how these cells affect tumor progression. We are focused in genetic and metabolic profiling that occur in tumor and MSCs.
Completed Projects
The effects of micro-environmental stress on the regenerative activity of mesenchymal stem cells DST-UKIERI |
“Effects of 20-HETE in mediating VEGF and NO induced changes in tissues by distinct signaling mechanisms-DST. |
Ongoing Projects
Centre of Excellence for Advanced Training and Research in Biomedical Applications -Tissue Engineering Osteoblasts and Human Dermal fibroblasts on PCL electrospun matrices sanctioned by MHRD. |
In vitro differentiation of mesenchymal stem cells into epithelial cell lineage - Clues from Gene profiling analysis-BUILDER PROGRAM. |
Invited Lectures
Delivered a Lecture on “A robust and scalable disease modeling and phenotypic drug screening platform for human cardiovascular disease using human induced pluripotent stem cells” at International Conference on Advances in Pharmaceutical and Health Sciences (ICAPHS-2020), Mangaluru, 20-22 Feb 2020. |
Delivered a webinar for CPD certification Sevice on “ Healthy Aging, neurodevelopment, cardiac regeneration and caloric restriction mimetics” conducted by the Aesthetic Multispeciality society, Spain. |
Delivered a guest lecture entitled , “Survey of Stem cell Technology in Indian Scenario” July 2020 in FDP on “ Evolving Trends in Biotechnology” conducted by Sri Venkateswara College of Engineering,Chennai, India. |
Delivered a short oral presentation “Bioactivation pathways in human Ovarian Follicular Fluid derived Mesenchymal Stem cells cultured on Chitosan/PCL/Zn Scaffold for Bone Tissue Regeneration” at XIth International Workshop on EPR in Biology and Medicine 6th to 10thOctober, 2019 in Krakow Poland. |
Delivered a talk at International Conference on Advances in Chemical Biology and Biologics (ICACB-2019), CSIR-IICT, Tarnaka, Hyderabad, 28 Feb - 02 Mar, 2019. |
Invited talk on “ My Journey through Stem cell Research-The yin and the Yang” in the Department of Biophysics, Medical College of Wisconsin, USA ,November 12th 2019. |
Invited talk on,” Identification and Characterisation of an Ideal biomaterial for Cardiac Tissue Engineering using Stem cell Technology” in National Conference on Emerging Trends in Smart Materials (NCETSM -18) conducted by SSN College of Engineering , January 19th, 2018 |
Delivered a Lecture on "Trends in Biotechnology- the Indian Scenario" in UK- India workshop on Industrial Biotechnology, organized by UK Science and Innovation Network and British Council,Chennai, 16th September,2016 |
Delivered a Lecture on "Curbing Cancer targeting the power house " in AMBICON organized by IMS and SUMS Hospital, Odisha (09-Jan-2016). |
Delivered a Lecture on "The Success story of Stem Cell Technology used for Pharmaceutical Technology " in Bio-Summit 2015 organized by Karpaga vinayaga college of Engineering & technology, Kanchipuram (08-Jan-2015). |
Delivered a Lecture on "Role of stem cells in the ageing endothelium" in ARCHA Symposium organized by Aston University, UK (16-Jul-2014). |
Delivered a Lecture on "Pitfalls in Regenerative Medicine-An Insight" in Future Trends in Biotechnology organized by PSG College of Technology, Coimbatore,India (03-May-2014). |
Delivered a Lecture on "Therapeutic application of bone marrow derived MScs cultured on bioscaffolds" in National Conference on current research perspective in Biotechnology, Healthcare & Environment organized by Dr. M.G.R. Educational and research Institute Univ, India (16-Apr-2013). |
Delivered a Lecture on "Biocompataibility studies of novel customizable polyamide scaffold for supporting osteoblast regeneration- Chances and challe" in Special Seminar in Department of Biophysics. organized by Medical college of Wisconsin, USA (12-Dec-2012). |
Delivered a Lecture on "Advances in Stem cell Research" in National seminar on Molecular Medicine organized by MepcoSchlenk, EngeeneringCollege,, Sivakasi,India (29-Aug-2012). |
Delivered a Lecture on "Whats in and out in Stem cell Technology" in National conference on Medicinal applications and advances. Organized by Aandal Alagar Engineenering College, Chennai (03-Jun-2012). |
Delivered a Lecture on "Advances in confocal Microscopy" in 23rd National congress of Parasitology organized by Indian society of Parasitology & Anna Univeristy, India (11-Nov-2011). |
Delivered a Lecture on "Recent trends in Bioscience- Advent & ambiguities" in Seminar organized by Dr. M.G.R. Educational and research institute Univ, India (29-Aug-2011). |
Delivered a Lecture on "Stem cells Boon or Bane" in Seminar on Biotechnology Uk and Indian Perspective organized by Centre for Biotechnology Anna University, India (11-Aug-2011). |
Delivered a Lecture on "Stem cell Technology" in Future perspectives in Biotechnology organized by Science City, Chennai., India (15-Dec-2010). |
Delivered a Lecture on "Novel Therapeutic approaches of Arachidonic acid derivatives" in Conducted Special Seminar on Department of Biochemistry organized by Sri Ramachandra Medical college and Research Insti, India (12-Jun-2009). |
Delivered a Lecture on "The RNA Avatars" in Seminar on Advanced Techniques in Biochemistry organized by Sri Ramachandra Medical college and Research Insti, India (10-May-2009). |
Membership and Positions in Professional Bodies
• Reviewer for Elsevier Publications
• Reviewer, PLOS journals
• Member of Animal Ethical Committee, Anna University
• Member of national screening committee for the selection of Fulbright-Nehru Post-Doctoral Grant applicants in the area of Science and Technology sponsored by USIEF
• Member of Japanese Biochemical Society
• Member of American Heart Association
• Expert Member, Institutional Committee for Stem Cell Research and Therapy (IC-SCRT,JIPMER, India
Research Guidance
Number of Ph.D Scholars Guided |
8 |
Number of Ph.D Scholars Guiding |
5 |
Number of M.S (By Research) Scholars Guided |
7 |
Number of M.Tech. Projects Guided |
31 |
Number of B.Tech. Projects Guided |
46 |
Number of PostDoctoral Fellow |
4 |
Selected Publications
|
All |
Citations |
2433 |
h-index |
24 |
i10-index |
41 |
No. of International Publications: 68
No. of National Publications : 3
No of Lectures Delivered in conferences, Guest Lectures and Invited Lectures: 74
- Vimalraj S, Subramanian R, Saravanan S, Arumugam B, Anuradha D. MicroRNA-432-5p regulates sprouting and intussusceptive angiogenesis in osteosarcoma microenvironment by targeting PDGFB. Lab Invest. 2021 Apr 12. doi: 10.1038/s41374-021-00589-3. Online ahead of print.PMID: 33846539
- Chandramohan Y, Jeganathan K, Sivanesan S, Koka P, Amritha TMS, Vimalraj S, Dhanasekaran A. Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration. Life Sci. 2021 Jan 1;264:118502. doi: 10.1016/j.lfs.2020.118502. Epub 2020 Oct 6.
- Pant T, Dhanasekaran A, Zhao M, Thorp EB, Forbess JM, Bosnjak ZJ, Benjamin IJ, Ge ZD. Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy. Sci Rep. 2021 Jan 28;11(1):2571. doi: 10.1038/s41598-021-82345-
- Vimalraj S, Saravanan S, Raghunandhakumar S, Anuradha D. Melatonin regulates tumor angiogenesis via miR-424-5p/VEGFA signaling pathway in osteosarcoma. Life Sci. 2020 Sep 1;256:118011. doi: 10.1016/j.lfs.2020.118011. Epub 2020 Jun 24.
- SeetaramanAmritha TM, Mahajan S, Subramaniam K, Chandramohan Y, Dhanasekaran A. Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.PLoS One. 2020 Nov 30;15(11):e0242798. doi: 10.1371/journal.pone.0242798. eCollection 2020.
- PraveenkumarSugumaran, Vishnusekar Narayanan, Daling Zhu, MeethaMedhora, Elizabeth Jacobs, Yamini Chandramohan, Vimalraj Selvaraj, Anuradha Dhanasekaran.Prophylactic supplementation of 20-HETE ameliorates hypoxia/reoxygenation injury in pulmonary vascular endothelial cells by inhibiting apoptosis. Acta Histochemica Volume 122, Issue 1, January 2020, 151461.
- Mayank G. JuheePandya,Divyang M. Patel, Ruturajsinh M. Vala, V. Ramkumar, Raghunandankumar Subramanian, Vivek K. Gupta, Ramesh L. Gardas, Anuradha Dhanasekaran,Hitendra M. Patel. One-Pot Assembly for Synthesis of 1, 4-Dihydropyridine Scaffold and Their Biological Applications. Journal of Polycyclic Aromatic Compounds. 2019 DOI: 10.1080/10406638.2019.1686401.
- Tarun Pant, Anuradha Dhanasekaran, Xiaowen Bai, Zeljko Bosnjak, Zhi-Dong Ge.Genome Wide Microarray Profiling Identified Differentially Expressed Circulating Biomarkers Associated with Early Development of Diabetic Cardiomyopathy. The FASEB Journal, Vol. 33, Pages 778.12 (2019).
- Hameed A, Natarajan M, Jabbar S, Dhanasekaran JJ, Kumar K, Sivanesan S, Kron M, Dhanasekaran A. Immune Response to BrugiamalayiAsparaginyl-tRNA Synthetase in Balb/c Mice and Human Clinical Samples of Lymphatic Filariasis. Lymphatic Research Biology. Vol. 17 (4),Pages 447-456 (2019).
- Selvaraj Vimalraj, Subramaniyam Rajalakshmi, Sekaran Saravanan, Deepak Thirumalai, MuruganKadarkarai, A Vasanthi Rajkumar, Anuradha Dhanasekaran. Zinc chelated morin promotes osteoblast differentiation over its uncomplexed counterpart. Process Biochemistry Vol. 82, Pages 167-172 (2019).
- Vimalraj S, Saravanan S, Anuradha D, Chatterjee S. Models to investigate intussusceptive angiogenesis: A special note on CRISPR/Cas9 based system in zebraf¬ish. Int J Biol Macromol. 2019 Feb 15;123:1229-1240.
- Pant T, Mishra MK, Bai X, Ge ZD, Bosnjak ZJ, Dhanasekaran A. Microarray analysis of long non-coding RNA and mRNA expression profiles in diabetic cardiomyopathy using human induced pluripotent stem cell-derived cardiomyocytes. Diabetic Vascular Diseases Research. 2019, Vol. 16(1), 57–68.
- Tarun Pant, Anuradha Dhanasekaran, Juan Fang, Xiaowen Bai, Zeljko Bosnjak, Mingyu Liang and Zhi Cong GE Current status and strategies of long non-coding RNA Research for Diabetic Cardiomyopathy. BMC Cardiovascular Disorders 2018 Oct 20; 18(1):197.
- Pavani Koka, Reddy Sailaja Mundre, Rohini Rangarajan, Yamini Chandramohan, Raghunandha Kumar Subramanian, Anuradha Dhanasekaran. Uncoupling Warburg effect and stemness in CD133+ve Cancer stem cells from Saos-2 (Osteosarcoma) cell line under hypoxia. Mol Biol Reports (2018)Volume 45, Issue 6, pp 1653–1662.
- Sekaran Saravanan, SelvarajVimalraj and Dhanasekaran Anuradha. Chitosan based thermoresponsive hydrogel containing graphene oxide for bone tissue repair. Biomedicine & Pharmacotherapy. Volume 107, July 2018, Pages 908-917
- Subramaniyam Rajalakshmi, Selvaraj Vimalraj, Sekaran Saravanan, Desingh Raj Preeth, ManickarajShairam and Dhanasekaran Anuradha Synthesis and characterization of silibinin/phenanthroline/ neocuproinecopper(II) complexes for augmenting bone tissue regeneration: an in vitro analysis.. Journal of Biological Inorganic Chemistry. March 2018. Jul;23(5):753-762
- Reddy Sailaja Mundre, Pavani Koka, Prakash Dhanaraj, Nitin Khatri, Sanjana Vig, Yamini Chandramohan and Anuradha Dhanasekaran Synergistic role of 5-azacytidine and ascorbic acid in directing cardiosphere derived cells to cardiomyocytes in vitro by downregulating Wnt signaling pathway via phosphorylation of β-catenin. November 30, 2017. Plos One.
- Ravikumar, A., P. Panneerselvam, K. Radhakrishnan, NorhashimahMorad, C. D. Anuradha, and S. Sivanesan. "DNAzyme based amplified biosensor on ultrasensitive fluorescence detection of Pb (II) ions from aqueous system." Journal of fluorescence 27, no. 6 (2017): 2101-2109.
- Bala, Raj Kiran, Rajkumar Murugesan, Sivanesan Subramanian, and Anuradha Dhanasekaran. "Auxin biosynthetic intermediate genes and their role in developmental growth and plasticity in higher plants." Journal of Plant Biochemistry and Biotechnology 26, no. 3 (2017): 321-329.
- Gokul Variar, Tarun Pant, Apoorva Singh, Abinaya Ravichandran, Sushant Swami, BalaramanKalyanaraman and Anuradha Dhanasekaran. Effect of mitochondrially targeted carboxy proxyl nitroxide on Akt-mediated survival in Daudi cells: Significance of a dual mode of action. April 2017. PLoS ONE 12(4): e0174546.
- M. A. Kumar, G. Vigneshwaran, M. E. Priya, M. Seenuvasan, V. V. Kumar, D. Anuradha, S. Sivanesan. Concocted bacterial consortium for the detoxification and mineralization of azoic-cum-sulfonic textile mill effluent. Journal of Water Process Engineering, 2017, 16, 199-205.
- Jeeva Jothi D, M Dhanraj, S Solaiappan, S Sivanesan, M Kron, A Dhanasekaran, "BrugiamalayiAsparaginyl-tRNA Synthetase Stimulates Endothelial Cell Proliferation, Vasodilation and Angiogenesis", PLOS ONE, published by PLOS ONE. Vol. 11, Issue 1, pp. 1-12 (2016).
- Samanta S, Balasubramanian S, Rajasingh S, Patel U, Dhanasekaran A, Dawn B, Rajasingh . MicroRNA: A new therapeutic strategy for cardiovascular diseases. J.Trends in cardiovascular medicine, March 2016. DOI: 10.1016/j.tcm.2016.02.004.
- Asiya Parvin A, Anuradha Dhanasekaran. Erythropoietin Regulation on p38 MAPK and Downstream targets of Akt during Hypoxia/Reperfusion injury on H9C2 cells. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6 (5) (2015).
- John E. Baker, JidongSu, Stacy Koprowski, Anuradha Dhanasekaran, Tom P. Aufderheide, and Garrett J. Gross, " Thrombopoietin Receptor Agonists Protect Human Cardiac Myocytes from Injury by Activation of Cell Survival Pathways", J Pharmacol Exp Ther, published by The American Society for Pharmacology and Experi. pp. 429-437 (2015).
- Asiya Parvin A, Rohini Rangarajan, Pavani Koka, Raj K Bala, Reddy Sailaja,MubeenFathima, Jeeva Jothi D, Sanjana Sivanesan, John E Baker, Anuradha Dhanasekaran, Recombinant Erythropoietin Mitigates Injury fromIschemia/Reperfusion in Neonatal Cardiomyocytes by Novel Multiple Signalling Pathways. Int J Pharm Pharm Sci (2015).
- Asiya Parvin A, John E Baker, Anuradha Dhanasekaran. Erythropoietin receptor mRNA expression in Human Cardiomyocytes. Journal of Biochemical Sciences, 2 (2015).
- A Parvin, U Shalini, A Devendran, JE Baker, A Dhanasekaran, "Erythropoietin protects cardiomyocytes from cell death during hypoxia/reperfusion injury through activation of survival signaling pathways", PLOS ONE, published by PLOS ONE. Vol. 9, Issue 9, pp. 1-12 (2014).
- ER Jacobs, S Bodiga, I Ali, AM Falck, JR Falck, M Medhora, A Dhanasekaran, " Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices", Experimental Cell Research, published by elsevier. Vol. 318, Issue 16, pp. 2143-2152 (2012).
- Anuradha DhanasekaranSreedhar Bodiga , Stephanie Gruenloh , Ying Gao , Laurel Dunn, John R. Falck , J. Noelle Buonaccorsi , MeethaMedhora , Elizabeth R. Jacobs, " 20-HETE increases survival and decreases apoptosis in pulmonary arteries and pulmonary artery endothelial cells", American Journal of Physiology-Heart and Circulatory Physiology , published by American Physiological Society. Vol. 296, Issue 3, pp. H777-H786 (2009).
- Anuradha Dhanasekaran , Stephanie K. Gruenloh , J. Noelle Buonaccorsi , Rong Zhang , Garrett J. Gross , John R. Falck , Paresh K. Patel , Elizabeth R. Jacobs , MeethaMedhora, "Multiple antiapoptotic targets of the PI3K/Akt survival pathway are activated by epoxyeicosatrienoic acids to protect cardiomyocytes from hypoxia/anoxia", American Journal of Physiology-Heart and Circulatory Physiology , published by American Physiological Society. Vol. 294, Issue 2, pp. H724-H735 (2008).
- MeethaMedhora ,Anuradha Dhanasekaran , Phillip F. Pratt Jr., Craig R. Cook , Laurel K. Dunn, Stephanie K. Gruenloh , Elizabeth R. Jacobs, " Role of JNK in network formation of human lung microvascular endothelial cells", American Journal of Physiology-Lung Cellular and Molecular Physiology , published by American Physiological Society. Vol. 294, Issue 4, pp. L676-L685 (2008).
- A Dhanasekaran, S Gruenloh, R Zhang, G Gross, J Falck, E Jacobs, M Medhora, "Epoxyeicosatrienoic acids (EETs) block cardiomyocyte cell death from hypoxia/reoxygenation injury via the PI3K/Akt survival pathway", FASEB, published by FASEB. Vol. 21, Issue 6, pp. A1288-A1288 (2007).
- A Dhanasekaran, R Al-Saghir, B Lopez, D Zhu, DD Gutterman, ER Jacobs, M Medhora, "Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature", American Journal of Physiology-Heart and Circulatory Physiology, published by American Physiological Society. Vol. 29, (2006).
- A Dhanasekaran, R Al-Saghir, B Lopez, D Zhu, DD Gutterman, ER Jacobs, M Medhora, "Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature", American Journal of Physiology-Heart and Circulatory Physiology, published by American Physiological Society. Vol. 29, (2006).
- A Dhanasekaran, S Gruenloh, E Jacobs, M Medhora, "Epoxyeicosatrienoic acids (EETs) protect cardiovascular cells from apoptosis mediated by caspase 3-dependent pathways", FASEB, published by FASEB. Vol. 20, Issue 4, pp. A123-A123 (2006).
- Anuradha Dhanasekaran, SrigiridharKotamraju, Chandran Karunakaran, Shasi V. Kalivendi, Simmy Thomas, Joy Joseph, B. Kalyanaraman, " Mitochondria superoxide dismutase mimetic inhibits peroxide-induced oxidative damage and apoptosis: role of mitochondrial superoxide", Free Radical Biology and Medicine , published by Elsevier. Vol. 39, Issue 5, pp. 567-583 (2005).
- Dhanasekaran A, Kotamraju S, Kalivendi SV, Matsunaga T, Shang T, Keszler A, Joseph J, Kalyanaraman B., "Supplementation of endothelial cells with mitochondria-targeted antioxidants inhibit peroxide-induced mitochondrial iron uptake, oxidative damage, and apoptosis", The journal of biological chemistry, published by JBC Papers. Vol. 279, Issue 36, pp. The journal of biological chemistry (2004).
- T Matsunaga, S Kotamraju, SV Kalivendi, A Dhanasekaran, J Joseph, B Kalyanaraman, "Ceramide-induced Intracellular Oxidant Formation, Iron Signaling, and Apoptosis in Endothelial Cells PROTECTIVE ROLE OF ENDOGENOUS NITRIC OXIDE", Journal of Biological Chemistry , published by y The American Society for Biochemistry and Molecu. Vol. 279, Issue 27,pp. 28614-28624 (2004).
- S Hirano, CD Anuradha, S Kanno, "krox20/egr2 is upregulated following non‐specific and homophilic adhesion in ratmacrophages", Immunology, Vol. 107, Issue 1, pp. 86-92 (2002).
- .S Kanno, CD Anuradha, S Hirano, "Localization of zinc after in vitro mineralization in osteoblastic cells", pp. springer (2001).
- CD Anuradha, S Kanno, S Hirano, " Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells", Free Radical Biology and Medicine, published by Elsevier. Vol. 31, Issue 3, pp. 367-373 (2001).
- S Kanno, CD Anuradha, S Hirano, " Chemotactic responses of osteoblastic MC3T3-E1 cells toward Zinc chloride. Biological Trace element research, Springer vol.83,issue1,pp49-55 (2001)
- CD Anuradha, S Kanno, S Hirano, "Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells", Archives of toxicology , published by Springer. Vol. 74, Issue 4, pp. 226-230 (2000).
- CD Anuradha, S Kanno, S Hirano, " RGD peptide-induced apoptosis in human leukemia HL-60 cells requires caspase-3 activation", Cell biology and toxicology , published by Springer. Vol. 16, Issue 5, pp. 275-283 (2000).
- S Hirano, CD Anuradha, S Kanno, " Transcription of krox-20/egr-2 is upregulated after exposure to fibrous particles and adhesion in rat alveolar macrophages", American journal of respiratory cell and molecular biology , published by Am Thoracic Soc. Vol. 23, Issue 3, pp. 313-319 (2000).