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Department of Biotechnology Anna University

Dr. Anuradha Dhanasekaran


Professor, Centre for Biotechnology

Anna University, Chennai 600025

044-22358363/8384

anu@annauniv.edu, anushivan@gmail.com
Director, Educational Multimedia Research Center

(EMMRC) Anna University, Chennai 600025

044-22358201/044-22300105

emmrcchennai@annauniv.edu

https://annauniv.edu/emmrc/







CORE COMPETENCIES


Comprehensive and in-depth knowledge in vascular biology, Redox Biology ,cardiovascular and stem cell biology. Developed techniques to isolate beating cardiomyocytes from rat pups and mastered isolation of stem cells from Umblical Cord, Bone marrow and cardiac explants.

Developed a CAM model to study angiogenesis and has developed Hypoxia models to study various therapeutic targets.

Discovered the anti-apoptotic property of EETS, leading to the development of possible drugs for myocardial Ischemia.



Previous Academic and Administrative Positions


Director, Centre for Biotechnology, Anna University, Chennai 600 025 May-2016 to April 2019.
Associate Professor, Centre for Biotechnology, Anna University, Chennai June-2012 to June 2015
Assistant Professor, Centre for Biotechnology, Anna University, Chennai December-2009 to May- 2012.
Deputy Director, Centre for International Affairs, Anna University, Chennai April-2013 to August-2013.
Additional Chief Coordinator , University Innovation Cluster Anna University July 2017-Till date
Chief Coordinator BUILDER program (Sponsored by DBT) May 2016-May 2019


Education

Degree Year University Major Subject Studied
M.Sc 1988-90 University of Madras Biochemistry
M.Phil 1990-91 University of Madras Biochemistry
PhD 1991-95 University of Madras Biochemistry

Post Doctoral Experience

Mar 1997-May 1998 Institute For Medical Microbiology and Hygiene, Johannes Gutenberg University, Mainz, GERMANY
Oct1998-Feb 2001 Regional Environment Division, National Institute for Environmental Studies, Tsukuba, JAPAN
Aug 2002-July 2004 Free Radical Research Center, Medical College Of Wisconsin, Milwaukee,Wisconsin, USA
Aug 2004-Aug 2009 Cardiovascular Research Center, Medical College Of Wisconsin, Milwaukee,Wisconsin, USA


Professional Experience


Completed Certificate course on Embryonic Stem cells isolation and characterization in the lab of Dr James Thompson who was the pioneer in isolating embryonic stem cells at the University of Wisconsin at Madison in Association with Wicell,USA
Worked on how Fluoride causes cell death at National Institute for Environmental Studies in Tsukuba, Japan.
Trained in the study of “Epigenetic Modifiers mediated targeting of microRNA in Human aortic endothelial cells at KU Cardiovascular Research Institute-The University of Kansas-USA.
Were trained in details of the Stem cell differentiation protocols that we use to efficiently and reproduciably differentiation Cardiomyocytes from pluripotent cells, at Department of Cell Biology, Neurobilogy & Anatomy-Medical College of Wisconsin,USA.
Worked in the capacity of Visiting Faculty conducted several experiments using Confocal Microscopy at Department of Radiation Oncology- Medical College of Wisconsin,USA.
Worked on the project involved introducing glycosylation sites in nanobodies by site directed mutagenesis at Department for Molecular Biomedical Research- University of Ghent, Ghent, Belgium
Underwent training in Instrumentation and Molecular biology techniques in National Research Council (CNR), Italy for two months
Attended a workshop on Molecular Oncology conducted by Cancer Biology Division of Madurai Kamaraj University, India and had  hands on training in all molecular biologytechniques
Worked as a Post doctoral fellow in Johannes Gutenberg University,Germany and worked on NF kappa B activation in response to acute toxins
Worked as a STA fellow at NIES, Japan on the gene expression of Krox-20 in response to adhesion in macrophages and apoptotic pathway in HL-60 cells on NaF exposure
Worked as a Post doctoral fellow in Free radical research Center Medical College of Wisconsin on the effect doxorubicin,an anticancer drug on BAEC cells and their intervention by mitochondrially targeted drugs –Mitoquinone and mitonitroxide.
Worked as Research Associate in Pulmonary and Critical Care Division,Medical College of Wisconsin on the protective role of eicosanoids in apoptosis and angiogenesis.


Membership and Positions in Professional Bodies


DBT Nominee as expert member for Animal Ethical Committee for several Institutions , IIT Madras, Apollo Hospitals to name a few.
Has been nominated as MEMBER, Project Advisory Committee for Bharathiar Cancer Theranostics Research Center, Bharatiyar University, Coimbatore, India.
Coordinator, DBT sponsored M.Tech Teaching Program May 2016- May 2019
Coordinator, DBT sponsored BTIS programme ,May 2016-May 2019
Coordinator, UGC assisted Special Assistance Program May 2016-May 2019
Reviewer of SERB projects,DST
ANNA UNIVERSITY NOMINEE for JRF recruitments at Anna University.
Member of national screening committee for the selection of Fulbright-Nehru Post-Doctoral Grant applicants in the area of Science and Technology sponsored by USIEF.
Member of the National Advisory Committee in the International conferences on Advances in Biotechnology and Biotherapeutics (ICABB-2017)
Nominated as an Expert Member, Board of Studies-Anna University –Thiruchirappalli.
Chairman/Member of Inspection Committee-Anna University Affiliation Colleges.
Coordinator/Member of Inspection Committee-Infrastructure facilities in the R&D Labs.
Member for the Departmental Consultative Committee (DCC).
Member of the Institutional Committee for Stem Cell Research &Therapy(IC-SCRT)-JIPMER, Puducherry.
Member of the Institutional Committee for Stem CellResearch&Therapy(IC-SCRT)- M.V.Diabetes Center, Chennai
Nominated as a Member of Selection Committee-Science City-Department of Higher Education, Government of Tamilnadu.
Member of Research Advisory Committee-Institute of Bio- Medical Research.
Member of the Board of Studies-Vivekanandha College of Engineering for Women. Tiruchengode.
Member of the Board of Studies -Karapagam Academy of Higher Education- Coimbatore.
Member of Board of Studies in various Colleges in Tamilnadu.
Expert Member, Review Team of Department of Scientific & Industrial Research(DSIR), Ministry of Science &Technology, Govt.of India, New Delhi.
Doctoral Advisory Committee-Academy of Scientific Innovative Research-CLRI.
Doctoral Committee Member-SRM University, VIT University, University of Madras and various other Insititutes.
External Subject Expert Viva –voce Examiner— IITs and Various other Institutions
Reviewer for Elsevier Publications and several reputed journals.
Member of Japanese Biochemical Society
Member of American Heart Association


ASSIGNMENTS ABROAD


Name of the Country Purpose Year Duration
Medical College of Wisconsin Milwaukee, USA Visiting Faculty November 2019 One month
Medical College of Wisconsin Milwaukee, USA Visiting Faculty July 2016 One month
University of Ghent, Ghent, Belgium Erasmus-Mundus Fellow April 2015 One month
Kansas University Medical Center Cardiovascular Research Institute –The University of Kansas USA Research Collaboration May 2014 One month
Aston University,Birmingham, United Kingdom Collaborative project under UKIERI scheme July 2014 One week
Medical college of Wisconsin-Department of Cell biology, Neurobiology Anatomy USA Research Collaboration August 2013 One month
Nigeria University Representative June 2013 Four days
Harbin Medical University, China Research Collaboration June 2012 Two months
Medical College of Wisconsin-Department of Radiation Oncology, USA Research Collaboration November 2012 One month
Cardiovascular Research Center, Medical College Of Wisconsin, Milwaukee,Wisconsin, USA Post-Doctoral Fellow Nov 2004-2009 Five years
Free Radical Research Center, Medical College Of Wisconsin, Milwaukee,Wisconsin, USA Post-Doctoral Fellow July 2002-2004 Two years
Regional Environment Division, National Institute for Environmental Studies, Tsukuba, JAPAN Post-Doctoral Fellow Feb 1998-2001 Three years
Institute For Medical Microbiology and Hygiene, Johannes Gutenberg University, Mainz, GERMANY Post-Doctoral Fellow May 1996-1997 One year
National Research Council (CNR), Italy Underwent training August 1995 Two months


360° Virtual Lab View








Areas of Expertise


• Tissue Engineering

• Angiogenesis

• Cell signalling

• Hypoxia

• Stem cell Technology

• Cardiovascular Therapy

• Cancer Biology







Stem cell Technology


• To create a platform that fosters inter-disciplinary collaborations toward bench to bedside translation of stem cell technologies for heart and vascular disease management

• To study the role of Wnt signaling in Stem cell differentiation

• Role of conditioned media from MScs

• Effects of micro-environmental stress on the regenerative activity of mesenchymal stem cells.

My lab focuses on further understanding of stem cell behavior, characterization, manipulation, expansion and therapeutic delivery

Cardiovascular Therapeutics


• In vitro studies in enhancing CSCs survival, proliferation and subsequent differentiation to functional Cardiomyocytes under hypoxic conditions.

• Analysis of anti-apoptotic and cyto protective pathways give clue regarding molecular mechanisms involved in decreasing the extent of cell death.

• Isolation, culture and characterization of CSCs from rat heart biopsies by explant method



Cancer Biology


• Understanding the signaling mechanisms in Cancer stem cells

• Mitochondrially targeted compounds novel therapeutic agents for Cancer novel therapeutic agents for Cancer

• Tumour Angiogenesis

Our goal is to understand the mechanism of tumor derived cytokines recruits and regulates the fate of infiltrating stem cells and how these cells affect tumor progression. We are focused in genetic and metabolic profiling that occur in tumor and MSCs.



Completed Projects


The effects of micro-environmental stress on the regenerative activity of mesenchymal stem cells DST-UKIERI
“Effects of 20-HETE in mediating VEGF and NO induced changes in tissues by distinct signaling mechanisms-DST.


Ongoing Projects


Centre of Excellence for Advanced Training and Research in Biomedical Applications -Tissue Engineering Osteoblasts and Human Dermal fibroblasts on PCL electrospun matrices sanctioned by MHRD.
In vitro differentiation of mesenchymal stem cells into epithelial cell lineage - Clues from Gene profiling analysis-BUILDER PROGRAM.


Invited Lectures


Delivered a Lecture on “A robust and scalable disease modeling and phenotypic drug screening platform for human cardiovascular disease using human induced pluripotent stem cells” at International Conference on Advances in Pharmaceutical and Health Sciences (ICAPHS-2020), Mangaluru, 20-22 Feb 2020.
Delivered a webinar for CPD certification Sevice on “ Healthy Aging, neurodevelopment, cardiac regeneration and caloric restriction mimetics” conducted by the Aesthetic Multispeciality society, Spain.
Delivered a guest lecture entitled , “Survey of Stem cell Technology in Indian Scenario” July 2020 in FDP on “ Evolving Trends in Biotechnology” conducted by Sri Venkateswara College of Engineering,Chennai, India.
Delivered a short oral presentation “Bioactivation pathways in human Ovarian Follicular Fluid derived Mesenchymal Stem cells cultured on Chitosan/PCL/Zn Scaffold for Bone Tissue Regeneration” at XIth International Workshop on EPR in Biology and Medicine 6th to 10thOctober, 2019 in Krakow Poland.
Delivered a talk at International Conference on Advances in Chemical Biology and Biologics (ICACB-2019), CSIR-IICT, Tarnaka, Hyderabad, 28 Feb - 02 Mar, 2019.
Invited talk on “ My Journey through Stem cell Research-The yin and the Yang” in the Department of Biophysics, Medical College of Wisconsin, USA ,November 12th 2019.
Invited talk on,” Identification and Characterisation of an Ideal biomaterial for Cardiac Tissue Engineering using Stem cell Technology” in National Conference on Emerging Trends in Smart Materials (NCETSM -18) conducted by SSN College of Engineering , January 19th, 2018
Delivered a Lecture on "Trends in Biotechnology- the Indian Scenario" in UK- India workshop on Industrial Biotechnology, organized by UK Science and Innovation Network and British Council,Chennai, 16th September,2016
Delivered a Lecture on "Curbing Cancer targeting the power house " in AMBICON organized by IMS and SUMS Hospital, Odisha (09-Jan-2016).
Delivered a Lecture on "The Success story of Stem Cell Technology used for Pharmaceutical Technology " in Bio-Summit 2015 organized by Karpaga vinayaga college of Engineering & technology, Kanchipuram (08-Jan-2015).
Delivered a Lecture on "Role of stem cells in the ageing endothelium" in ARCHA Symposium organized by Aston University, UK (16-Jul-2014).
Delivered a Lecture on "Pitfalls in Regenerative Medicine-An Insight" in Future Trends in Biotechnology organized by PSG College of Technology, Coimbatore,India (03-May-2014).
Delivered a Lecture on "Therapeutic application of bone marrow derived MScs cultured on bioscaffolds" in National Conference on current research perspective in Biotechnology, Healthcare & Environment organized by Dr. M.G.R. Educational and research Institute Univ, India (16-Apr-2013).
Delivered a Lecture on "Biocompataibility studies of novel customizable polyamide scaffold for supporting osteoblast regeneration- Chances and challe" in Special Seminar in Department of Biophysics. organized by Medical college of Wisconsin, USA (12-Dec-2012).
Delivered a Lecture on "Advances in Stem cell Research" in National seminar on Molecular Medicine organized by MepcoSchlenk, EngeeneringCollege,, Sivakasi,India (29-Aug-2012).
Delivered a Lecture on "Whats in and out in Stem cell Technology" in National conference on Medicinal applications and advances. Organized by Aandal Alagar Engineenering College, Chennai (03-Jun-2012).
Delivered a Lecture on "Advances in confocal Microscopy" in 23rd National congress of Parasitology organized by Indian society of Parasitology & Anna Univeristy, India (11-Nov-2011).
Delivered a Lecture on "Recent trends in Bioscience- Advent & ambiguities" in Seminar organized by Dr. M.G.R. Educational and research institute Univ, India (29-Aug-2011).
Delivered a Lecture on "Stem cells Boon or Bane" in Seminar on Biotechnology Uk and Indian Perspective organized by Centre for Biotechnology Anna University, India (11-Aug-2011).
Delivered a Lecture on "Stem cell Technology" in Future perspectives in Biotechnology organized by Science City, Chennai., India (15-Dec-2010).
Delivered a Lecture on "Novel Therapeutic approaches of Arachidonic acid derivatives" in Conducted Special Seminar on Department of Biochemistry organized by Sri Ramachandra Medical college and Research Insti, India (12-Jun-2009).
Delivered a Lecture on "The RNA Avatars" in Seminar on Advanced Techniques in Biochemistry organized by Sri Ramachandra Medical college and Research Insti, India (10-May-2009).


Membership and Positions in Professional Bodies


• Reviewer for Elsevier Publications

• Reviewer, PLOS journals

• Member of  Animal Ethical Committee, Anna University

• Member of national screening committee for the selection of Fulbright-Nehru Post-Doctoral Grant applicants in the area of Science and Technology sponsored by USIEF

• Member of Japanese Biochemical Society

• Member of American Heart Association

• Expert Member, Institutional Committee for Stem Cell Research and Therapy (IC-SCRT,JIPMER, India


Research Guidance


Number of Ph.D Scholars Guided 8
Number of Ph.D Scholars Guiding 5
Number of M.S (By Research) Scholars Guided 7
Number of M.Tech. Projects Guided 31
Number of B.Tech. Projects Guided 46
Number of PostDoctoral Fellow 4






Selected Publications


All
Citations 2433
h-index 24
i10-index 41

No. of International Publications: 68


No. of National Publications : 3


No of Lectures Delivered in conferences, Guest Lectures and Invited Lectures: 74


  • Vimalraj S, Subramanian R, Saravanan S, Arumugam B, Anuradha D. MicroRNA-432-5p regulates sprouting and intussusceptive angiogenesis in osteosarcoma microenvironment by targeting PDGFB. Lab Invest. 2021 Apr 12. doi: 10.1038/s41374-021-00589-3. Online ahead of print.PMID: 33846539
  • Chandramohan Y, Jeganathan K, Sivanesan S, Koka P, Amritha TMS, Vimalraj S, Dhanasekaran A. Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration. Life Sci. 2021 Jan 1;264:118502. doi: 10.1016/j.lfs.2020.118502. Epub 2020 Oct 6.
  • Pant T, Dhanasekaran A, Zhao M, Thorp EB, Forbess JM, Bosnjak ZJ, Benjamin IJ, Ge ZD. Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy. Sci Rep. 2021 Jan 28;11(1):2571. doi: 10.1038/s41598-021-82345-
  • Vimalraj S, Saravanan S, Raghunandhakumar S, Anuradha D. Melatonin regulates tumor angiogenesis via miR-424-5p/VEGFA signaling pathway in osteosarcoma. Life Sci. 2020 Sep 1;256:118011. doi: 10.1016/j.lfs.2020.118011. Epub 2020 Jun 24.
  • SeetaramanAmritha TM, Mahajan S, Subramaniam K, Chandramohan Y, Dhanasekaran A. Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.PLoS One. 2020 Nov 30;15(11):e0242798. doi: 10.1371/journal.pone.0242798. eCollection 2020.
  • PraveenkumarSugumaran, Vishnusekar Narayanan, Daling Zhu, MeethaMedhora, Elizabeth Jacobs, Yamini Chandramohan, Vimalraj Selvaraj, Anuradha Dhanasekaran.Prophylactic supplementation of 20-HETE ameliorates hypoxia/reoxygenation injury in pulmonary vascular endothelial cells by inhibiting apoptosis. Acta Histochemica Volume 122, Issue 1, January 2020, 151461.
  • Mayank G. JuheePandya,Divyang M. Patel, Ruturajsinh M. Vala, V. Ramkumar, Raghunandankumar Subramanian, Vivek K. Gupta, Ramesh L. Gardas, Anuradha Dhanasekaran,Hitendra M. Patel. One-Pot Assembly for Synthesis of 1, 4-Dihydropyridine Scaffold and Their Biological Applications. Journal of Polycyclic Aromatic Compounds. 2019 DOI: 10.1080/10406638.2019.1686401.
  • Tarun Pant, Anuradha Dhanasekaran, Xiaowen Bai, Zeljko Bosnjak, Zhi-Dong Ge.Genome Wide Microarray Profiling Identified Differentially Expressed Circulating Biomarkers Associated with Early Development of Diabetic Cardiomyopathy. The FASEB Journal, Vol. 33, Pages 778.12 (2019).
  • Hameed A, Natarajan M, Jabbar S, Dhanasekaran JJ, Kumar K, Sivanesan S, Kron M, Dhanasekaran A. Immune Response to BrugiamalayiAsparaginyl-tRNA Synthetase in Balb/c Mice and Human Clinical Samples of Lymphatic Filariasis. Lymphatic Research Biology. Vol. 17 (4),Pages 447-456 (2019).
  • Selvaraj Vimalraj, Subramaniyam Rajalakshmi, Sekaran Saravanan, Deepak Thirumalai, MuruganKadarkarai, A Vasanthi Rajkumar, Anuradha Dhanasekaran. Zinc chelated morin promotes osteoblast differentiation over its uncomplexed counterpart. Process Biochemistry Vol. 82, Pages 167-172 (2019).
  • Vimalraj S, Saravanan S, Anuradha D, Chatterjee S. Models to investigate intussusceptive angiogenesis: A special note on CRISPR/Cas9 based system in zebraf¬ish. Int J Biol Macromol. 2019 Feb 15;123:1229-1240.
  • Pant T, Mishra MK, Bai X, Ge ZD, Bosnjak ZJ, Dhanasekaran A. Microarray analysis of long non-coding RNA and mRNA expression profiles in diabetic cardiomyopathy using human induced pluripotent stem cell-derived cardiomyocytes. Diabetic Vascular Diseases Research. 2019, Vol. 16(1), 57–68.
  • Tarun Pant, Anuradha Dhanasekaran, Juan Fang, Xiaowen Bai, Zeljko Bosnjak, Mingyu Liang and Zhi Cong GE Current status and strategies of long non-coding RNA Research for Diabetic Cardiomyopathy. BMC Cardiovascular Disorders 2018 Oct 20; 18(1):197.
  • Pavani Koka, Reddy Sailaja Mundre, Rohini Rangarajan, Yamini Chandramohan, Raghunandha Kumar Subramanian, Anuradha Dhanasekaran. Uncoupling Warburg effect and stemness in CD133+ve Cancer stem cells from Saos-2 (Osteosarcoma) cell line under hypoxia. Mol Biol Reports (2018)Volume 45, Issue 6, pp 1653–1662.
  • Sekaran Saravanan, SelvarajVimalraj and Dhanasekaran Anuradha. Chitosan based thermoresponsive hydrogel containing graphene oxide for bone tissue repair. Biomedicine & Pharmacotherapy. Volume 107, July 2018, Pages 908-917
  • Subramaniyam Rajalakshmi, Selvaraj Vimalraj, Sekaran Saravanan, Desingh Raj Preeth, ManickarajShairam and Dhanasekaran Anuradha Synthesis and characterization of silibinin/phenanthroline/ neocuproinecopper(II) complexes for augmenting bone tissue regeneration: an in vitro analysis.. Journal of Biological Inorganic Chemistry. March 2018. Jul;23(5):753-762
  • Reddy Sailaja Mundre, Pavani Koka, Prakash Dhanaraj, Nitin Khatri, Sanjana Vig, Yamini Chandramohan and Anuradha Dhanasekaran Synergistic role of 5-azacytidine and ascorbic acid in directing cardiosphere derived cells to cardiomyocytes in vitro by downregulating Wnt signaling pathway via phosphorylation of β-catenin. November 30, 2017. Plos One.
  • Ravikumar, A., P. Panneerselvam, K. Radhakrishnan, NorhashimahMorad, C. D. Anuradha, and S. Sivanesan. "DNAzyme based amplified biosensor on ultrasensitive fluorescence detection of Pb (II) ions from aqueous system." Journal of fluorescence 27, no. 6 (2017): 2101-2109.
  • Bala, Raj Kiran, Rajkumar Murugesan, Sivanesan Subramanian, and Anuradha Dhanasekaran. "Auxin biosynthetic intermediate genes and their role in developmental growth and plasticity in higher plants." Journal of Plant Biochemistry and Biotechnology 26, no. 3 (2017): 321-329.
  • Gokul Variar, Tarun Pant, Apoorva Singh, Abinaya Ravichandran, Sushant Swami, BalaramanKalyanaraman and Anuradha Dhanasekaran. Effect of mitochondrially targeted carboxy proxyl nitroxide on Akt-mediated survival in Daudi cells: Significance of a dual mode of action. April 2017. PLoS ONE 12(4): e0174546.
  • M. A. Kumar, G. Vigneshwaran, M. E. Priya, M. Seenuvasan, V. V. Kumar, D. Anuradha, S. Sivanesan. Concocted bacterial consortium for the detoxification and mineralization of azoic-cum-sulfonic textile mill effluent. Journal of Water Process Engineering, 2017, 16, 199-205.
  • Jeeva Jothi D, M Dhanraj, S Solaiappan, S Sivanesan, M Kron, A Dhanasekaran, "BrugiamalayiAsparaginyl-tRNA Synthetase Stimulates Endothelial Cell Proliferation, Vasodilation and Angiogenesis", PLOS ONE, published by PLOS ONE. Vol. 11, Issue 1, pp. 1-12 (2016).
  • Samanta S, Balasubramanian S, Rajasingh S, Patel U, Dhanasekaran A, Dawn B, Rajasingh . MicroRNA: A new therapeutic strategy for cardiovascular diseases. J.Trends in cardiovascular medicine, March 2016. DOI: 10.1016/j.tcm.2016.02.004.
  • Asiya Parvin A, Anuradha Dhanasekaran. Erythropoietin Regulation on p38 MAPK and Downstream targets of Akt during Hypoxia/Reperfusion injury on H9C2 cells. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6 (5) (2015).
  • John E. Baker, JidongSu, Stacy Koprowski, Anuradha Dhanasekaran, Tom P. Aufderheide, and Garrett J. Gross, " Thrombopoietin Receptor Agonists Protect Human Cardiac Myocytes from Injury by Activation of Cell Survival Pathways", J Pharmacol Exp Ther, published by The American Society for Pharmacology and Experi. pp. 429-437 (2015).
  • Asiya Parvin A, Rohini Rangarajan, Pavani Koka, Raj K Bala, Reddy Sailaja,MubeenFathima, Jeeva Jothi D, Sanjana Sivanesan, John E Baker, Anuradha Dhanasekaran, Recombinant Erythropoietin Mitigates Injury fromIschemia/Reperfusion in Neonatal Cardiomyocytes by Novel Multiple Signalling Pathways. Int J Pharm Pharm Sci (2015).
  • Asiya Parvin A, John E Baker, Anuradha Dhanasekaran. Erythropoietin receptor mRNA expression in Human Cardiomyocytes. Journal of Biochemical Sciences, 2 (2015).
  • A Parvin, U Shalini, A Devendran, JE Baker, A Dhanasekaran, "Erythropoietin protects cardiomyocytes from cell death during hypoxia/reperfusion injury through activation of survival signaling pathways", PLOS ONE, published by PLOS ONE. Vol. 9, Issue 9, pp. 1-12 (2014).
  • ER Jacobs, S Bodiga, I Ali, AM Falck, JR Falck, M Medhora, A Dhanasekaran, " Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices", Experimental Cell Research, published by elsevier. Vol. 318, Issue 16, pp. 2143-2152 (2012).
  • Anuradha DhanasekaranSreedhar Bodiga , Stephanie Gruenloh , Ying Gao , Laurel Dunn, John R. Falck , J. Noelle Buonaccorsi , MeethaMedhora , Elizabeth R. Jacobs, " 20-HETE increases survival and decreases apoptosis in pulmonary arteries and pulmonary artery endothelial cells", American Journal of Physiology-Heart and Circulatory Physiology , published by American Physiological Society. Vol. 296, Issue 3, pp. H777-H786 (2009).
  • Anuradha Dhanasekaran , Stephanie K. Gruenloh , J. Noelle Buonaccorsi , Rong Zhang , Garrett J. Gross , John R. Falck , Paresh K. Patel , Elizabeth R. Jacobs , MeethaMedhora, "Multiple antiapoptotic targets of the PI3K/Akt survival pathway are activated by epoxyeicosatrienoic acids to protect cardiomyocytes from hypoxia/anoxia", American Journal of Physiology-Heart and Circulatory Physiology , published by American Physiological Society. Vol. 294, Issue 2, pp. H724-H735 (2008).
  • MeethaMedhora ,Anuradha Dhanasekaran , Phillip F. Pratt Jr., Craig R. Cook , Laurel K. Dunn, Stephanie K. Gruenloh , Elizabeth R. Jacobs, " Role of JNK in network formation of human lung microvascular endothelial cells", American Journal of Physiology-Lung Cellular and Molecular Physiology , published by American Physiological Society. Vol. 294, Issue 4, pp. L676-L685 (2008).
  • A Dhanasekaran, S Gruenloh, R Zhang, G Gross, J Falck, E Jacobs, M Medhora, "Epoxyeicosatrienoic acids (EETs) block cardiomyocyte cell death from hypoxia/reoxygenation injury via the PI3K/Akt survival pathway", FASEB, published by FASEB. Vol. 21, Issue 6, pp. A1288-A1288 (2007).
  • A Dhanasekaran, R Al-Saghir, B Lopez, D Zhu, DD Gutterman, ER Jacobs, M Medhora, "Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature", American Journal of Physiology-Heart and Circulatory Physiology, published by American Physiological Society. Vol. 29, (2006).
  • A Dhanasekaran, R Al-Saghir, B Lopez, D Zhu, DD Gutterman, ER Jacobs, M Medhora, "Protective effects of epoxyeicosatrienoic acids on human endothelial cells from the pulmonary and coronary vasculature", American Journal of Physiology-Heart and Circulatory Physiology, published by American Physiological Society. Vol. 29, (2006).
  • A Dhanasekaran, S Gruenloh, E Jacobs, M Medhora, "Epoxyeicosatrienoic acids (EETs) protect cardiovascular cells from apoptosis mediated by caspase 3-dependent pathways", FASEB, published by FASEB. Vol. 20, Issue 4, pp. A123-A123 (2006).
  • Anuradha Dhanasekaran, SrigiridharKotamraju, Chandran Karunakaran, Shasi V. Kalivendi, Simmy Thomas, Joy Joseph, B. Kalyanaraman, " Mitochondria superoxide dismutase mimetic inhibits peroxide-induced oxidative damage and apoptosis: role of mitochondrial superoxide", Free Radical Biology and Medicine , published by Elsevier. Vol. 39, Issue 5, pp. 567-583 (2005).
  • Dhanasekaran A, Kotamraju S, Kalivendi SV, Matsunaga T, Shang T, Keszler A, Joseph J, Kalyanaraman B., "Supplementation of endothelial cells with mitochondria-targeted antioxidants inhibit peroxide-induced mitochondrial iron uptake, oxidative damage, and apoptosis", The journal of biological chemistry, published by JBC Papers. Vol. 279, Issue 36, pp. The journal of biological chemistry (2004).
  • T Matsunaga, S Kotamraju, SV Kalivendi, A Dhanasekaran, J Joseph, B Kalyanaraman, "Ceramide-induced Intracellular Oxidant Formation, Iron Signaling, and Apoptosis in Endothelial Cells PROTECTIVE ROLE OF ENDOGENOUS NITRIC OXIDE", Journal of Biological Chemistry , published by y The American Society for Biochemistry and Molecu. Vol. 279, Issue 27,pp. 28614-28624 (2004).
  • S Hirano, CD Anuradha, S Kanno, "krox20/egr2 is upregulated following non‐specific and homophilic adhesion in ratmacrophages", Immunology, Vol. 107, Issue 1, pp. 86-92 (2002).
  • .S Kanno, CD Anuradha, S Hirano, "Localization of zinc after in vitro mineralization in osteoblastic cells", pp. springer (2001).
  • CD Anuradha, S Kanno, S Hirano, " Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells", Free Radical Biology and Medicine, published by Elsevier. Vol. 31, Issue 3, pp. 367-373 (2001).
  • S Kanno, CD Anuradha, S Hirano, " Chemotactic responses of osteoblastic MC3T3-E1 cells toward Zinc chloride. Biological Trace element research, Springer vol.83,issue1,pp49-55 (2001)
  • CD Anuradha, S Kanno, S Hirano, "Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells", Archives of toxicology , published by Springer. Vol. 74, Issue 4, pp. 226-230 (2000).
  • CD Anuradha, S Kanno, S Hirano, " RGD peptide-induced apoptosis in human leukemia HL-60 cells requires caspase-3 activation", Cell biology and toxicology , published by Springer. Vol. 16, Issue 5, pp. 275-283 (2000).
  • S Hirano, CD Anuradha, S Kanno, " Transcription of krox-20/egr-2 is upregulated after exposure to fibrous particles and adhesion in rat alveolar macrophages", American journal of respiratory cell and molecular biology , published by Am Thoracic Soc. Vol. 23, Issue 3, pp. 313-319 (2000).


Significant Papers Presented in Conferences


  • K. Naveena, D. Naveena and C.D. Anuradha. Identification of novel lysyl hydroxylase inhibitors and their molecular insights – A computational approach. 5th IITM - Tokyo Tech Joint Symposium on "Current trends in Bioinformatics: Big data analysis, machine learning and drug design" at IIT Madras, Chennai, March 06-07, 2020.
  • J. Kavya, C. Yamini, S. Vimalraj, Pavani koka, S. Raghunandha Kumar, R. Murali and C.D. Anuradha. Enhancement of Osteoblast differentiation of Folllicular fluid derived Mesenchymal stem cells by zinc coated chitosan/PCL scaffold. International Conference on “Horizon 2019: The epitome of biomedical research” at Saveetha Dental College, Chennai, Feb 28 – Mar 01, 2019.
  • S. Gokul Krishna, S. Raghunandha Kumar, Pavani Koka and C.D. Anuradha. Invivo tracking of graphene nanoparticles tagged mesenchymal stem cells for cell based therapies. International Conference on “Horizon 2019: The epitome of biomedical research” at Saveetha Dental College, Chennai, Feb 28 – Mar 01, 2019.
  • R. Nikarika, S. Raghunandha Kumar, C. Yamini, Pavani Koka, Hitendra M. Patel and C.D. Anuradha. Identification of novel Mcl-1 inhibitor and anticancer activity of 1,4-dihydropyridine derivative against pro/anti-apoptotic pathway in Non-small cell lung cancer (A549) cells. 3rd PAN IIT BIOTECH MEET 2019 on “Cancer Precision Meicine and Personalized Therapeutics” at IIT Madras, Chennai, Jan 31 - Feb 2, 2019.
  • C. Rithika, N, Mahalakshmi, K. Naveena, Michael Kron and C.D. Anuradha. Evaluation od recombinant Brugiamalayiasparaginyl tRNA synthetase variants for angiogenic properties on endothelial cells. International Conference on “Advances in Therapeutic Molecules and Drug Design” at Karpagam Academy of Higher Education, Coimbatore, Jan 9-11, 2019.
  • Sathyamoorthy Balasubramanian, J. Thangavel, K.N.Dileepan, A.Dhanasekaran, B.Dawn and J.Rajasingh. Combinatorial treatment with epigenetic modifiers inhibits thrombin- induced eNOS/RhoA signaling and restores AJ integrity. Experimental Biology Annual meeting 2014, April 26-30 2014, San Diego , USA.
  • Jeeva Jothi Dhanasekaran, S.Solaiyappan, M. Dhanraj, S.Chatterjee, M.Kron and Anuradha Dhanasekaran. Expression and characterization of Asparagine- tRNA synthetase: the key enzyme that mediates angiogenesis in lymphatic filariasis. Experimental Biology Annual meeting 2014, April 26-30 2014, San Diego , USA
  • Rohini Rangarajan and Anuradha Dhanasekaran. Comparison and characterization of PVA/Vanillin scaffolds for tissue engineering applications. 12-14 March 2014, Vellore Institute of Technology, Vellore,India.
  • Abinayaa Ravichandran, Gokul Variar, Tarun Pant, Apoorva Singh and Anuradha Dhanasekaran. Hypoxia-induced apoptosis in HL-60 cells. A prelude to treatment with targeted compounds. 30th Jan-  1st Feb 2014, Cancercon, IIT Madras, India.
  • AsiyaParvinAllaudeen, AjayDevendran. John E Baker, Anuradha Dhanasekaran. Recombinant Human Erythropoietin Prevents Reactive Oxygen Species  - Induced Apoptosis Via  Akt and p38 MAPK Pathway During Hypoxia/ Reperfusion Injury.Basic Cardiovascular Sciences 2013 .American Heart Association- Circulation Research. Las Vegas,Nevada,USA July, 2013
  • ThiruvarutChelvan S, Johnson W E Anuradha Dhanasekaran. Role of secretion of MSCs in presence of Leptin. National conference on Biolife.Sanjay Gandhi Postgraduate Institute of Medical Sciences,Lucknow, India ,March 2013
  • Ruban Rex P, Johnson W E, Arumaikkannu G Anuradha Dhanasekaran. Analysis of a novel customizable polyamide scaffold to support osteoblast differentiationInvitro bio-compatability study. National conference on Biolife.Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.4,5th March 2013.
  • JeevaJothi D, SuvroChattergiee, Anuradha Dhanasekaran. Ap3A inhibits AsnRs-induced angiogenesis in Ehy926 endothelial cells. International workshop on wound healing & angiogenesis AU-KBC Research Centre, Chennai, India, December 2013
  • AsiyaParvin .A, Raj Pranp, Shalini.U Anuradha Dhanasekaran. “Erythropoietin receptor signaling in cardiomyocytes protection during hypoxia   reperfusion injury” along with National conference on “Impact of Biotechnology in Health care & Industry Vel Tech High Tech Dr.Rangarajan, Dr. Sakunthala Engineering College, Avadi, Chennai, India, 2011
  • JeevaJothi.D, Anuradha Dhanasekaran ‘Expression and purification of Asperhine-tRNA synthetases in lymphatic filariasis’, 23rd National congress on Parasitology,Anna University,  Chennai, India 2011
  • C.D.Anuradha,Kanno,S Seishiro Hirano RGD peptide- induced apoptosis in Human leukemia HL-60 cells requires caspase-3 activation 18th International Conference of Biochemistry and Molecular Biology (IUBMB) Birmingham, UK,July 2000
  • C.D.Anuradha, Kanno,S Seishiro Hirano Cytochrome-c release and caspase activation in NaF-induced apoptosis in HL-60 cells Gordon Research Conference-Mechanisms of Toxicity New Hampshire, USA, July 2000
  • C.D.Anuradha, Kanno,S Seishiro Hirano Fluoride induces apoptosis in HL-60 cells through a mechanism involving oxidative stress Gordon Research Conference-Oxidative stress and Diseases Los Angeles, USA, March 2001
  • C.D.Anuradha,Kanno,S Seishiro Hirano Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride induced apoptosis in HL-60 cells 40thSociety of Toxicology Meeting San Fransisco, USA, March 2001.
  • C.D.Anuradha,Kanno,S Seishiro Hirano Fluoride induces apoptosis in Human leukemia (HL-60) cells by down regulation of Bcl-2 ASBMB meeting on Experimental Biology Orlando, USA, April 2001.
  • C.D.Anuradha, SrigiridharKotamraju, Joseph J, Murphy MP, Kalyanaraman(2003):Supplementation of endothelial cells with mitochondrially-targeted ubiquinone derivative, MitoQ    inhibits H2O2-induced mitochondrial iron uptake and apoptosis Society for Free radicals in Biology and Medicine. Seattle, USA, Nov 2003
  • Anuradha Dhanasekaran, Stephanie K. Gruenloh, Elizabeth R. Jacobs and MeethaMedhora(2005). EETs protect microvascular endothelial cells from Fas-induced apoptosis. American Heart Association. Texas,USA 2005
  • Anuradha Dhanasekaran, Stephanie K. Gruenloh, Elizabeth R. Jacobs and MeethaMedhora (2005). EETs protect cardiomyocytes from hypoxia-reperfusion induced injury by a AKT-dependent pathway. FASEB meeting. San Fransisco, USA 2006.
  • Anuradha Dhanasekaran, Stephanie K. Gruenloh, Rong Zhang, Noelle Buonaccorsi, Elizabeth R. Jacobs and MeethaMedhora(2007) PI3K/Akt pathway is activated by epoxyeicosatrienoic acids (EETs) to protect cardiomyocytes from apoptosis induced by hypoxia/reoxygenation injury. GORDON RESEARCH CONFERENCE‐Oxidative stress and Diseases.Ventura Beach,California 2007.

Conferences Organised


  • Organizer, International conference on "Avidadham-2016- An International conference on Recent Advances in Diagnostics and Treatment of Metabolic Disorders “from March 18 &19-2016.
  • Organizer, One day Seminar on Stem Cells in Tissue Regeneration-Organized by Anna University & Aston University-DST-UKIERI, On October 2015.
  • Organizer, International level conference on "Avidadham-2015 International conference on Contemporary Research Trends in Diagnostics and Therapeutics" from 16-Feb-2015 to 18-Feb-2015.
  • Organiser, International level conference on "Avidadham-2014- An International conference on frontiers and challenges in healthcare, Biotechnology and food processing Technology"from 03-Feb-2014 to 05-Feb-2014.
  • Organizing Secretary, International level conference on "Avidadham- 13 International level conference on Molecular therapeutics organized by Anna University.
  • Organizer, International level seminar on "Biotechnology: UK and Indian Perspectives" from 13-Oct-2011 to 14-Oct-2011.
  • Organizing Secretary, National level conference on "National congress of Parasitology, jointly organized by the Indian Society for Parasitology and Anna University" November-2011.

Special Eminent Lectures Organised


  • Organised a lecture on October 12th, 2012 by Nobel Laureate Jules A Hoffman,who won Nobel prize in Physiology and Medicine ,2011 who delivered a lecture on Host defense mechanism and Innate Immunity in association with Science and Technology Division, French Embassy ,India..
  • Organised lecture in Anna University by American Immunologist Prof. Max Cooper who has won various prestigious awards including the Lasker award for his discovery of B lymphocytes, – a monumental achievement that provided the organizing principle of the adaptive immune system and launched the course of modern immunology
  • MHRD – GLOBAL INITIATIVE FOR ACADEMIC NETWORKS (GIAN) COURSE ORGANIZED
    1. Targeting Cancer Metabolism [166020H04] – by Dr. Balaraman Kalyanaraman from 05.12.2016 -09.12.2016 at Centre for Biotechnology, Anna University, Chennai. https://gian.iitkgp.ac.in//files/brochures/BR1470898080Raman_brochure.pdf
    2. An Insight to Infectious diseases [166020H03] – by Dr. Michael Kron from 05.02.2017 – 09.02.2017 at Centre for Biotechnology, Anna University, Chennai.https://gian.iitkgp.ac.in//files/brochures/BR1484031141Dr_kron.pdf
    3. Cell Redox biology and its Therapeutics – by Dr. Jeanette Vasquez Vivar from 16.07.2018 – 20.07.2018 at Centre for Biotechnology, Anna University, Chennai.https://gian.iitkgp.ac.in//files/brochures/BR1524459829GIAN_CellRedox.pdf
    4. Biomedical imaging: Advanced FLIM - FRET Microscopy Techniques for Cancer Cellular Imaging-By Dr Ammasi Periyasamy from 17th to 22nd December 2018 https://gian.iitkgp.ac.in//files/brochures/BR1540294344aruna_medphy.pdf
    5. Experimental Models in Stem Cells and Tissue Regeneration [176020HO1]by Eustace WJohnson from 16th to 27th September 2019. https://gian.iitkgp.ac.in//files/brochures/BR1561959379experimental.pdf
    6. Advances in Radiation Biology by Meetha M. Medhora, from 14th to 18th October 2019 at Centre for Biotechnology, Anna University, Chennai. https://gian.iitkgp.ac.in//files/brochures/BR1561959422advances.pdf

Collaborators


BALARAMAN KALYANARAMAN, PhD
Chairman and Professor of Biophysics
Medical College of Wisconsin
USA
ELIZABETH ROGERS JACOBS, MD
Professor and Chief Pulmonary Medicine
Medical College of Wisconsin
USA
MICHAEL KRON, MD, MS
Dept of Medicine
Medical college of Wisconsin
USA
Dr MEETHA MEDHORA
Professor,
Radiation Oncology,
Medical College of Wisconsin
USA
JOHN E. BAKER PhD
Professor,
Department of Surgery
Division of Cardiothoracic
Medical College of Wisconsin
USA
RAJASINGH JOHNSON
Division of Cardiovascular Diseases
Kansas Medical Center Kansas
USA
EUSTACE JOHNSON PhD
Life and Health Sciences
Aston University
UK




Awards


  • Tamilnadu State Award ( TANSA ) for best Scientist in “Medical Sciences” for 2018.
  • Prof. M. VishwanathanHonour award for outstanding research awarded by M.V. Diabetes Research Centre and Hospitals, Chennai (2017).
  • “Best Researcher Award” for outstanding contributions awarded by Center for Technology development and transfer, Anna University, India (2015).
  • Was selected to attend the Management and Leadership progamme conducted jointly by CMI in association with Dudley College of London and AICTE, India.
  • Was awarded CMI Level 5 certification in Management and Leadership by the Royal Charter,United Kingdom.
  • Six GIAN (Global Initiative for Academic Network) programs proposed were approved by MHRD/Ministry of Education,India.
    https://gian.iitkgp.ac.in/cgenmenu/objectives
    https://www.annauniv.edu/gian/index.html
  • Visiting Fellow at Ghent University, Belgium March-April 2015 under SVAGATA Fellowship. Recipient of Svagata fellowship (2014-15) under the Erasmus Mundus Action 2 (EMA2)
  • "International Conference Travel grant" given by Anna University from India (2013).
  • Has been cited in Asia's Who's Who of Men & Women of Achievement (2010).
  • Got the “MOST CITED PAPER AWARD” from Elsevier publications for the year (2005-2007).
  • Got the “BEST RESEARCH PRESENTATION AWARD” for best research presentation by the Department of Medicine, Medical College of Wisconsin during Research Day 2005.
  • Young Scientist Travel Award was awarded to present at the ASBMB meeting on Experimental Biology in Orlando, USA, April 2001
  • Travel Award from the chair’s fund to present at the GORDON RESEARCH CONFERENCE – Mechanisms of Toxicity held in Newhampshire, Boston, July 2000
  • The American Society for Biochemistry and Molecular Biology Postdoctoral Travel Award program at Orlando, Florida in March 2001.
  • JSPS Fellow Science and Technology Agency Award by Japan Science and Technology Corporation and Japan International Science and Technology Exchange Center to work as a researcher in Japan in August-2000-2001.
  • Received Young Scientist awardby Biochemical Society and FEBS and presented a paper in the 18th International Conference of Biochemistry and Molecular Biology ( IUBMB) in Birmingham,UK, July 2000.
  • The research work done in Japan was considered as the pioneering work and as a breakthrough in fluoride toxicity studies and was cited in the Mercola news letter. The article is posted at: http://mercola.com/2000/sep/24/fluoride_kills_cells.htm
  • Anuradha CD et al: RGD peptide-induced apoptosis in Human leukemia HL-60 cells requires caspase-3 activation. Presented at the 18th International Conference of Biochemistry and Molecular Biology (IUBMB), Birmingham, UK, July 2000. This work was considered to be cutting edge research and was published as a special note in the Biochemist magazine.
  • Has been cited in “Whos Who in Science in Technology “ in 2000
  • Science and Technology Agency Award by Japan Science and Technology Corporation and Japan International Science and Technology Exchange Center to work as a researcher in Japan in September 1998-2000.






Contact


Professor, Centre for Biotechnology


Anna University, Chennai 600025


044-22358363/8384


anu@annauniv.edu, anushivan@gmail.com

Director, Educational Multimedia Research Center


(EMMRC) Anna University, Chennai 600025


044-22358201/044-22300105


emmrcchennai@annauniv.edu


https://annauniv.edu/emmrc/


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